Evaluation of Energy Balance on Human Telomerase Reverse Transcriptase (hTERT) Alternative Splicing by Semi-quantitative RT-PCR in Human Umbilical Vein Endothelial Cells
نویسندگان
چکیده
منابع مشابه
Evaluation of Energy Balance on Human Telomerase Reverse Transcriptase (hTERT) Alternative Splicing by Semi-quantitative RT-PCR in Human Umbilical Vein Endothelial Cells
BACKGROUND Decreased high-energy phosphate level is involved in endothelial cell injury and dysfunction. Reduced telomerase activity in endothelial cells in parallel with reduced energy levels might be due to altered direction of alternative splicing machine as a complication of depleted energy during the process of atherosclerosis. MATERIALS AND METHODS Isolated human umbilical vein endothel...
متن کاملExpression Pattern of Alternative Splicing Variants of Human Telomerase Reverse Transcriptase (hTERT) in Cancer Cell Lines Was not Associated with the Origin of the Cells
Telomerase and systems controlling their activity have been of great attention. There are controversies regarding the role of the alternative splicing forms of the human telomerase reverse transcriptase (hTERT), the catalytic subunit of telomerase. Therefore, the correlation between telomerase enzyme activity, the abundance of alternatively spliced variants of hTERT and doubling time of a seri...
متن کاملImmunotherapy of tumors with human telomerase reverse transcriptase immortalized human umbilical vein endothelial cells.
Human umbilical endothelial cells (HUVECs) have been proven to be effective in tumor anti-angiogenesis but the mechanism remained to be further demonstrated. The restricted ability of HUVECs to proliferate in vitro also limits their application on a large scale. In the present study, we immortalized HUVECs with hTERT genes by lentiviral infection and explored the antitumor immunity of hTERT-exp...
متن کاملexpression pattern of alternative splicing variants of human telomerase reverse transcriptase (htert) in cancer cell lines was not associated with the origin of the cells
telomerase and systems controlling their activity have been of great attention. there are controversies regarding the role of the alternative splicing forms of the human telomerase reverse transcriptase (htert), the catalytic subunit of telomerase. therefore, the correlation between telomerase enzyme activity, the abundance of alternatively spliced variants of htert and doubling time of a serie...
متن کاملHuman telomerase reverse transcriptase (hTERT) gene expression in thyroid neoplasms.
Ten percent of fine-needle aspirations (FNAs) of the thyroid are deemed "indeterminate" or "suspicious" for malignancy by the cytopathologist, but most of these lesions are benign. Therefore, additional markers of malignancy may prove to be a useful adjunct. The catalytic component of telomerase, human telomerase reverse transcriptase (hTERT), has been found to be reactivated in immortalized ce...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Advanced Biomedical Research
سال: 2017
ISSN: 2277-9175
DOI: 10.4103/2277-9175.204591